Antioxidant Activity and Hemolytic Effect of Hydro-methanolic Extract and its Phenolic Enriched Fractions From Leaves and Stems of Salvia officinalis L. from Algeria

This study aimed to assess an in vitro antioxidant and hemolytic activities of crude hydromethanolic extract, ethyl acetate n-butanol fractions from leaves stems Salvia officinalis L. Extract prepared by maceration S. were determined for their phytochemical composition contents total phenolic flavonoid. Antioxidant activity was evaluated the methods DPPH radical scavenging, ferric reducing power capacity. Hemolysis assay carried out evaluate toxicity studied samples. Crude extract contain different secondary metabolites considerable flavonoids. They exhibit high low effect. However, fraction is characterized highest (362.75 ± 0.07 µg GAE/mg DE) flavonoid (263.27 0.1 CE/mg contents. Furthermore, this shows best tested DPPH, FRAP TAC assays. The antiradical against (IC50 = 208.51 5.77 μg/mL) close that ascorbic acid 206.43 4.16 μg/mL). It exhibits a powerful reduce iron (EC50 250 0.001 capacity (817.33 0.06 reveals lowest hemolysis rate 3.77 0.02% with 200 mg/mL fraction, after one hour incubation. obtained results suggest are considered efficient natural antioxidants safe human erythrocytes.
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